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	Provedor de dados Trop. Plant Pathol. (2) Autor Accotto,Gian Paolo (1) Colariccio,Addolorata (1) FAJARDO,THOR V.M. (1) JELKMANN,WILHELM (1) KUHN,GILMAR B. (1) Lovisolo,Osvaldo (1) Masenga,Vera (1) NICKEL,OSMAR (1) Mais... Palavra-chave Capillovirus (2) RT-PCR (2) Citrus tatter leaf virus (1) Molecular characterization (1) Tipo do documento Info:eu-repo/semantics/article (1) Info:eu-repo/semantics/other (1) Ano 2003 (1) 2001 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última An isolate of Apple stem grooving virus associated with Cleopatra mandarin fruit intumescence Trop. Plant Pathol. Lovisolo,Osvaldo; Accotto,Gian Paolo; Masenga,Vera; Colariccio,Addolorata. A citrus tatter leaf isolate (CTLV-Cl) of Apple stem grooving virus (ASGV) has been found to be associated with a fruit rind intumescence in Cleopatra mandarin (Citrus reshni) in Limeira (SP). The CTLV-Cl was mechanically transmitted to the main experimental herbaceous hosts of CTLV. Chenopodium quinoa and C. amaranticolor reacted with local lesions and systemic symptoms while other test plants reacted somewhat differently than what is reported for CTLV. A pair of primers designed for specific detection of ASGV and CTLV amplified the expected 801 bp fragment from the CTLV-Cl-infected plants. Typical capillovirus-like particles were observed by the electron microscope in experimentally infected C. quinoa and C. amaranticolor leaves. Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus tatter leaf virus; Capillovirus; RT-PCR. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582003000100008 Sequence analysis of the capsid protein gene of an isolate of Apple stem grooving virus, and its survey in Southern Brazil Trop. Plant Pathol. NICKEL,OSMAR; FAJARDO,THOR V.M.; JELKMANN,WILHELM; KUHN,GILMAR B.. Apple stem grooving virus (ASGV) is one of the most important viruses infecting fruit trees. This study aimed at the molecular characterization of ASGV infecting apple (Malus domestica) plants in Santa Catarina (SC). RNA extracted from plants infected with isolate UV01 was used as a template for RT-PCR using specific primers. An amplified DNA fragment of 755 bp was sequenced. The coat protein gene of ASGV isolate UV01 contains 714 nucleotides, coding for a protein of 237 amino acids with a predicted Mr of approximately 27 kDa. The nucleotide and the deduced amino acid sequences of the coat protein gene showed identities of 90.9% and 97.9%, respectively, with a Japanese isolate of ASGV. Very high amino acid homologies (98.7%) were also found with Citrus... Tipo: Info:eu-repo/semantics/other Palavras-chave: Molecular characterization; Capillovirus; RT-PCR. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000300014 Registros recuperados: 2 Primeira ... 1 ... Última

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